Safe water supply, sanitation facilities and safe environments are the priorities of the communities in the villages of Upper Egypt. However, personal hygienic behaviors need improvement for substantial promotion of family health. This cannot be met without the full participation of women in their various roles in their communities. Accordingly, 375 females belonging to rural villages of Egypt (VHVs) volunteered to have a role in health and sanitation aspects and disseminate hygienic messages to their communities. The objective of this study was to assess and upgrade the levels of awareness, attitude and practice of VHVs towards environmental sanitation, sanitary housing, personal hygiene, safe water, food safety, sanitary sewage and refuse disposals. Implementation of environmental health education courses resulted in significant improvement of VHVs' awareness, attitude and practice towards the majority of the environmental topics that were addressed. Similar extensive courses are recommended for the promotion of environmental sanitation in other communities.