Single-step DNA immobilization on antifouling self-assembled monolayers covalently bound to silicon (111)

Till Böcking; Kristopher A Kilian; Katharina Gaus; J Justin Gooding

doi:10.1021/la060331a

Single-step DNA immobilization on antifouling self-assembled monolayers covalently bound to silicon (111)

Langmuir. 2006 Apr 11;22(8):3494-6. doi: 10.1021/la060331a.

Authors

Till Böcking¹, Kristopher A Kilian, Katharina Gaus, J Justin Gooding

Affiliation

¹ School of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia.

PMID: 16584219
DOI: 10.1021/la060331a

Abstract

Hydrosilylation of alkenes with epoxide-terminated tri(ethylene oxide) moieties on Si-H surfaces yields homogeneous monolayers for the efficient coupling of biomolecules. The wetting properties of the epoxide-functionalized surface allow for the spotting of solutions of biomolecules, making the surface amenable to microarraying. Immobilization of thiolated DNA was achieved in a single step to fabricate biorecognition interfaces showing the hybridization of complementary DNA at low concentrations and negligible binding of noncomplementary DNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkenes / chemistry
Base Sequence
DNA / chemistry*
Epoxy Compounds / chemistry
Hydrogen / chemistry*
Hydrogen-Ion Concentration
Models, Chemical
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis*
Oxides / chemistry
Semiconductors
Silanes / chemistry
Silicon / chemistry*
Sulfhydryl Compounds / chemistry
Surface Properties

Substances

Alkenes
Epoxy Compounds
Oxides
Silanes
Sulfhydryl Compounds
Hydrogen
DNA
Silicon