Variable expression patterns of Mycobacterium tuberculosis PE_PGRS genes: evidence that PE_PGRS16 and PE_PGRS26 are inversely regulated in vivo

Veerabadran Dheenadhayalan; Giovanni Delogu; Maurizio Sanguinetti; Giovanni Fadda; Michael J Brennan

doi:10.1128/JB.188.10.3721-3725.2006

Variable expression patterns of Mycobacterium tuberculosis PE_PGRS genes: evidence that PE_PGRS16 and PE_PGRS26 are inversely regulated in vivo

J Bacteriol. 2006 May;188(10):3721-5. doi: 10.1128/JB.188.10.3721-3725.2006.

Authors

Veerabadran Dheenadhayalan¹, Giovanni Delogu, Maurizio Sanguinetti, Giovanni Fadda, Michael J Brennan

Affiliation

¹ Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.

Abstract

Evaluation of expression of 16 PE_PGRS genes present in Mycobacterium tuberculosis under various growth conditions demonstrated constitutive expression of 7 genes, variable expression of 7 genes, and no expression of 2 genes. An inverse expression profile for genes PE_PGRS16 and PE_PGRS26 was observed to occur in macrophages and in mice infected with M. tuberculosis. Variable expression of PE_PGRS proteins could have implications for their role in the immunopathogenesis of tuberculosis.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Antigens, Bacterial / genetics*
Bacterial Proteins / genetics*
Gene Expression Regulation, Bacterial*
Genetic Variation*
Membrane Proteins / genetics*
Mycobacterium tuberculosis / genetics*
Mycobacterium tuberculosis / growth & development
Protein Isoforms / genetics

Substances

Antigens, Bacterial
Bacterial Proteins
Membrane Proteins
PE-PGRS protein, Mycobacterium
Protein Isoforms