Chemokine IL-8 and chemokine receptor CXCR3 and CXCR4 gene expression in childhood acute lymphoblastic leukemia at first relapse

J Pediatr Hematol Oncol. 2006 Apr;28(4):216-20. doi: 10.1097/01.mph.0000212908.14642.a5.

Abstract

In this study, we examined the gene expression of interleukin (IL)-8, CXCR3, and CXCR4 in leukemic cells from 100 children with relapsed B-cell progenitors (BCP) acute lymphoblastic leukemia (ALL), using quantitative real-time polymerase chain reaction (RT-PCR). IL-8, CXCR3, and CXCR4 were expressed in almost all bone marrow (BM) samples. The CXCR4 expression significantly correlated with known prognostic factors at relapse: time point and site of relapse. Patients who had a combined BM relapse (n=21) had lower IL-8 and CXCR4 expression than those who had an isolated BM relapse (n=79). The CXCR3 expression was higher in female patients (n=39) than in male patients (n=61). However, this did not reach prognostic relevance in relapsed ALL.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Marrow / pathology*
  • Child
  • DNA Primers
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Interleukin-8 / genetics*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / immunology
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / isolation & purification
  • Receptors, CXCR3
  • Receptors, CXCR4 / genetics*
  • Receptors, Chemokine / genetics*
  • Recurrence
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • CXCR3 protein, human
  • DNA Primers
  • Interleukin-8
  • RNA, Neoplasm
  • Receptors, CXCR3
  • Receptors, CXCR4
  • Receptors, Chemokine