Abstract
Human TRIM5alpha (TRIM5alpha(hu)) potently restricts N-tropic (N-MLV), but not B-tropic, murine leukemia virus in a manner dependent upon residue 110 of the viral capsid. Rhesus monkey TRIM5alpha (TRIM5alpha(rh)) inhibits N-MLV only weakly. The study of human-monkey TRIM5alpha chimerae revealed that both the v1 and v3 variable regions of the B30.2/SPRY domain contain potency determinants for N-MLV restriction. These variable regions are predicted to be surface-exposed elements on one face of the B30.2 domain. Acidic residues in v3 complement basic residue 110 of the N-MLV capsid. The results support recognition of the retroviral capsid by the TRIM5alpha B30.2 domain.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Antigens, Viral / drug effects
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Antigens, Viral / immunology*
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Antiviral Restriction Factors
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Capsid / physiology
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Carrier Proteins / pharmacology
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Humans
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Leukemia Virus, Murine / chemistry
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Leukemia Virus, Murine / drug effects*
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Leukemia Virus, Murine / pathogenicity
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Protein Structure, Tertiary
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Proteins / chemistry
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Proteins / pharmacology
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Retroviridae Infections / physiopathology
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Retroviridae Infections / prevention & control*
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Tripartite Motif Proteins
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Ubiquitin-Protein Ligases
Substances
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Antigens, Viral
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Antiviral Restriction Factors
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Carrier Proteins
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Proteins
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Tripartite Motif Proteins
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TRIM5 protein, human
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TRIM5(alpha) protein, rhesus monkey
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Ubiquitin-Protein Ligases