The kinetics of reactive uptake of N2O5 on submicron aerosol particles containing humic acid and ammonium sulfate has been investigated as a function of relative humidity (RH) and aerosol composition using a laminar flow reactor coupled with a differential mobility analyzer (DMA) to characterize the aerosol. For single-component humic acid aerosol the uptake coefficient, gamma, was found to increase from 2 to 9 x 10(-4) over the range 25-75% RH. These values are 1-2 orders of magnitude below those typically observed for single-component sulfate aerosols (Phys. Chem. Chem. Phys. 2003, 5, 3453-3463;(1) Atmos. Environ. 2000, 34, 2131-2159(2)). For the mixed aerosols, gamma was found to decrease with increasing humic acid mass fraction and increase with increasing RH. For aerosols containing only 6% humic acid by dry mass, a decrease in reactivity of more than a factor of 2 was observed compared with the case for single-component ammonium sulfate. The concentration of liquid water in the aerosol droplets was calculated using the aerosol inorganic model (for the ammonium sulfate component) and a new combined FTIR-DMA system (for the humic acid component). Analysis of the uptake coefficients using the water concentration data shows that the change in reactivity cannot be explained by the change in water content alone. We suggest that, due to its surfactant properties, the main effect of the humic acid is to reduce the mass accommodation coefficient for N2O5 at the aerosol particle surface. This has implications for the use of particle hygroscopicity data for predictions of the rate of N2O5 hydrolysis.