IL-2-induced IL-13 production by allergen-specific human helper T cell clones

Int Arch Allergy Immunol. 2006:140 Suppl 1:51-4. doi: 10.1159/000092711. Epub 2006 Jun 9.

Abstract

Background: Interleukin (IL)-5 and IL-13 are important cytokines in allergic diseases such as asthma and atopic dermatitis. We have reported that the production of IL-5 and IL-13 by mite-responsive T helper cells (Th) is controlled under similar signal requirements, but precise mechanisms are not yet well characterized.

Methods: Allergen-specific Th clones were established from peripheral blood lymphocytes of atopic asthmatics, and cytokine synthesis in response to various stimuli was determined by specific ELISAs. IL-13 gene expression was enumerated by quantitative real-time PCR.

Results: IL-13 production was clearly induced by IL-2. IL-13 mRNA expression was also induced.

Conclusion: The IL-2 signal by itself causes IL-13 synthesis independent of T cell receptor stimulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / immunology*
  • Antigens, Dermatophagoides / immunology*
  • Cells, Cultured
  • Clone Cells
  • Enzyme-Linked Immunosorbent Assay
  • Gene Expression
  • Gene Expression Regulation / immunology
  • Humans
  • Interleukin-13 / biosynthesis*
  • Interleukin-13 / immunology
  • Interleukin-2 / immunology
  • Interleukin-2 / metabolism*
  • Lymphocyte Activation / immunology
  • Receptors, Antigen, T-Cell / immunology
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocytes, Helper-Inducer / immunology*

Substances

  • Allergens
  • Antigens, Dermatophagoides
  • Interleukin-13
  • Interleukin-2
  • Receptors, Antigen, T-Cell