Abstract
We assessed the ability of a kanamycin-marked Stx phage to move into a commensal, ovine Escherichia coli strain in the ruminant gastrointestinal tract. Transduction was detected in 19/24 sheep tested, resulting in the recovery of 47 transductants. Subtherapeutic doses of the quinolone antibiotic enrofloxacin did not increase the rate of transduction.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Coliphages / genetics*
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Coliphages / physiology
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Escherichia coli / genetics
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Escherichia coli / virology
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Escherichia coli O157 / genetics
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Escherichia coli O157 / virology
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Gastrointestinal Tract / microbiology
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Ruminants / microbiology
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Sheep / microbiology*
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Shiga Toxin / genetics*
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Transduction, Genetic*