Absence of the full-length breast cancer-associated gene-1 leads to increased expression of insulin-like growth factor signaling axis members

Cancer Res. 2006 Jul 15;66(14):7151-7. doi: 10.1158/0008-5472.CAN-05-4570.

Abstract

The breast cancer-associated gene-1 (BRCA1) plays many important functions in multiple biological processes/pathways. Mice homozygous for a targeted deletion of full-length BRCA1 (Brca1Delta11/Delta11) display both increased tumorigenesis and premature aging, yet molecular mechanisms underlying these defects remain elusive. Here, we show that Brca1 deficiency leads to increased expression of several insulin-like growth factor (IGF) signaling axis members in multiple experimental systems, including BRCA1-deficient mice, primary mammary tumors, and cultured human cells. Furthermore, we provide evidence that activation of IGF signaling by BRCA1 deficiency can also occur in a p53-independent fashion. Our data indicate that BRCA1 interacts with the IRS-1 promoter and inhibits its activity that is associated with epigenetic modification of histone H3 and histone H4 to a transcriptional repression chromatin configuration. We further show that BRCA1-deficient mammary tumor cells exhibit high levels of IRS-1, and acute suppression of Irs-1 using RNA interference significantly inhibits growth of these cells. Those observations provide a molecular insight in understanding both fundamental and therapeutic BRCA1-associated tumorigenesis and aging.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • BRCA1 Protein / deficiency*
  • BRCA1 Protein / genetics
  • Genes, BRCA1*
  • Genes, p53
  • Humans
  • Insulin Receptor Substrate Proteins
  • Insulin-Like Growth Factor I / biosynthesis*
  • Mammary Glands, Animal / metabolism
  • Mammary Glands, Animal / physiology
  • Mammary Neoplasms, Experimental / genetics*
  • Mammary Neoplasms, Experimental / metabolism
  • Mice
  • Phosphoproteins / genetics
  • Promoter Regions, Genetic
  • RNA Interference
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Signal Transduction
  • Transfection

Substances

  • BRCA1 Protein
  • IRS1 protein, human
  • Insulin Receptor Substrate Proteins
  • Irs1 protein, mouse
  • Phosphoproteins
  • RNA, Messenger
  • Insulin-Like Growth Factor I