Computational prediction of cis-regulatory modules from multispecies alignments using Galaxy, Table Browser, and GALA

Methods Mol Biol. 2006:338:91-103. doi: 10.1385/1-59745-097-9:91.

Abstract

One major goal of genomics is to identify all the functional sequences in genomes, including sequences that regulate the expression of genes. Sequence conservation is a good, albeit imperfect, guide to these functional elements. We describe how to use publicly available servers (Galaxy, the UCSC Table Browser, and GALA) to find genomic sequences whose alignments (from blastZ and multiZ) show properties associated with cis-regulatory modules, such as high conservation score, high regulatory potential score, and conserved transcription factor binding sites. Links to these servers can be accessed at http://www.bx.psu.edu/ and http://genome.ucsc.edu/.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Conserved Sequence
  • Databases, Genetic
  • Genes, Regulator
  • Genomics / statistics & numerical data
  • Humans
  • Internet
  • Sequence Alignment / statistics & numerical data*
  • Software*
  • Species Specificity