Genome-scale loss-of-function screening with a lentiviral RNAi library

David E Root; Nir Hacohen; William C Hahn; Eric S Lander; David M Sabatini

doi:10.1038/nmeth924

Genome-scale loss-of-function screening with a lentiviral RNAi library

Nat Methods. 2006 Sep;3(9):715-9. doi: 10.1038/nmeth924.

Authors

David E Root¹, Nir Hacohen, William C Hahn, Eric S Lander, David M Sabatini

Affiliation

¹ Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA. droot@broad.mit.edu

PMID: 16929317
DOI: 10.1038/nmeth924

Abstract

The discovery that RNA interference (RNAi) is functional in mammalian cells led us to form The RNAi Consortium (TRC) with the goal of enabling large-scale loss-of-function screens through the development of genome-scale RNAi libraries and methodologies for their use. These resources form the basis of a loss-of-function screening platform created at the Broad Institute. Our human and mouse libraries currently contain >135,000 lentiviral clones targeting 27,000 genes. Initial screening efforts have demonstrated that these libraries and methods are practical and powerful tools for high-throughput lentivirus RNAi screens.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Gene Expression Regulation / genetics*
Gene Library*
Genetic Vectors*
Humans
Lentivirus / genetics*
Mice
RNA, Small Interfering / genetics*

Substances

RNA, Small Interfering