Infection of monocytes/macrophages by HIV in vitro

Res Virol. 1990 Mar-Apr;141(2):225-31. doi: 10.1016/0923-2516(90)90025-e.

Abstract

Because of the very important role of the mononuclear phagocyte system in the immunopathogenesis of HIV infection, a culture system for in vitro studies of infection of monocytes/macrophages with HIV was developed. A method is described for the infection of human monocytes/macrophages cultured on hydrophobic membranes (Teflon) with different strains of HIV. The HIV isolates can be characterized according to their replication potential on monocytes/macrophages cultures. The biological properties of some HIV1 and HIV2 isolates are compared in lymphocyte and monocyte/macrophage cultures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acquired Immunodeficiency Syndrome / immunology
  • Acquired Immunodeficiency Syndrome / pathology*
  • HIV-1 / growth & development*
  • HIV-2 / growth & development
  • Humans
  • In Vitro Techniques
  • Macrophages / immunology
  • Macrophages / microbiology*
  • Macrophages / ultrastructure
  • Membranes, Artificial
  • Monocytes / immunology
  • Monocytes / microbiology*
  • Monocytes / ultrastructure
  • Polytetrafluoroethylene
  • RNA-Directed DNA Polymerase / metabolism
  • Virus Cultivation

Substances

  • Membranes, Artificial
  • Polytetrafluoroethylene
  • RNA-Directed DNA Polymerase