Expression of the murine interleukin-5 receptor on Xenopus laevis oocytes

Biochem Biophys Res Commun. 1990 Oct 30;172(2):570-5. doi: 10.1016/0006-291x(90)90711-u.

Abstract

In this study we describe the use of Xenopus laevis oocytes for the detection of mRNA coding for a murine interleukin-5 (mI15) receptor. When injected with sucrose gradient fractionated polyA+ RNA derived from the murine 115-dependent pre B cell line B13, these oocytes could specifically bind 35S-methionine labeled mI15. A size of approximately 4000 nucleotides (25S) was estimated for the mRNA corresponding to the mIL5-binding activity. This binding was not blocked by a monoclonal antibody R52 specific for the MI15-receptor, suggesting that the oocytes express a different form of this receptor.

MeSH terms

  • Animals
  • Cell Line
  • Interleukin-5 / metabolism
  • Kinetics
  • Mice
  • Microinjections
  • Oocytes / metabolism*
  • Poly A / administration & dosage
  • Poly A / genetics
  • Poly A / isolation & purification
  • RNA / administration & dosage
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger
  • Receptors, Immunologic / genetics*
  • Receptors, Immunologic / metabolism
  • Receptors, Interleukin*
  • Receptors, Interleukin-5
  • Recombinant Proteins / metabolism
  • Transcription, Genetic
  • Xenopus laevis

Substances

  • Interleukin-5
  • RNA, Messenger
  • Receptors, Immunologic
  • Receptors, Interleukin
  • Receptors, Interleukin-5
  • Recombinant Proteins
  • Poly A
  • RNA