Structure determination of VEGF-E by sulfur SAD

Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1430-4. doi: 10.1107/S0907444906036742. Epub 2006 Oct 18.

Abstract

The crystal structure of VEGF-E was solved by the sulfur single-wavelength anomalous dispersion method (S-SAD) using highly redundant low-resolution data collected at a wavelength of lambda approximately 1.7 A with an estimated anomalous signal of 1.5%. 11 sulfur sites, nine out of 16 disulfide bonds and two out of 12 methionines could be located in the asymmetric unit using data truncated at a resolution of 4.1 A; however, none of the common diffraction data-quality indicators for SAD allowed clear discrimination between successful and unsuccessful resolution cutoffs. The high solvent content of 75% allowed efficient density modification to be performed and an unbiased electron-density map of good quality to be generated. This study demonstrates the strength of S-SAD for phasing using accurate highly redundant data at low resolution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crystallography, X-Ray / methods
  • Disulfides / chemistry*
  • Humans
  • Models, Molecular*
  • Protein Structure, Tertiary
  • Sulfur / chemistry*
  • Vascular Endothelial Growth Factor A / chemistry*

Substances

  • Disulfides
  • Vascular Endothelial Growth Factor A
  • Sulfur