Adipose tissue induction in vivo

Adv Exp Med Biol. 2006:585:403-12. doi: 10.1007/978-0-387-34133-0_26.

Abstract

Engineering adipogenic tissue in vivo requires the concomitant induction of angiogenesis to generate a stable long-term three-dimensional construct. Histioconductive tissue engineering strategies have been used. The disadvantage of using biodegradable scaffolds is a delayed angiogenic induction resulting in ischemic necrosis of the central cell population in the scaffold. We evaluated an histioinductive approach for adipose tissue engineering by combining essential key components for adipogenic induction: (1) a precursor cell source, (2) a vascular pedicle, (3) a supportive matrix, and (4) a chamber to preserve space for the new tissue to develop. We observed concomitant adipogenic and angiogenic induction after 6 weeks in three-dimensional adipose tissue constructs.

Publication types

  • Review

MeSH terms

  • Adipose Tissue / pathology*
  • Animals
  • Biocompatible Materials / chemistry
  • Collagen / chemistry
  • Drug Combinations
  • Extracellular Matrix / metabolism
  • Guided Tissue Regeneration
  • Humans
  • Imaging, Three-Dimensional
  • Laminin / chemistry
  • Mesenchymal Stem Cells / cytology
  • Mice
  • Muscle, Skeletal / cytology
  • Neoplasm Transplantation
  • Neovascularization, Pathologic
  • Proteoglycans / chemistry
  • Time Factors
  • Tissue Engineering / methods*

Substances

  • Biocompatible Materials
  • Drug Combinations
  • Laminin
  • Proteoglycans
  • matrigel
  • Collagen