Abstract
We report a methylcytosine-selective fluorescence labeling. An amino-modified bipyridine was prepared and used as an osmium ligand for methylcytosine-selective oxidation. Different fluorophores were easily incorporated to the modified methylcytosine site through an amide formation of the ligand amino group after osmium complexation.
MeSH terms
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2,2'-Dipyridyl / analogs & derivatives*
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2,2'-Dipyridyl / chemical synthesis
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2,2'-Dipyridyl / chemistry
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5-Methylcytosine / analysis*
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Fluorescent Dyes / chemistry*
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Oligodeoxyribonucleotides / chemistry
Substances
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Fluorescent Dyes
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N-(4-aminobutyl)-5-(2-(4-methylpyridin-2-yl)pyridin-4-yl)pentanamide
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Oligodeoxyribonucleotides
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2,2'-Dipyridyl
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5-Methylcytosine