Abstract
Dendritic cells (DCs) enhance human immunodeficiency virus type 1 (HIV-1) infection of CD4(+) T lymphocytes in trans. The C-type lectin DC-SIGN, expressed on DCs, binds to the HIV-1 envelope glycoprotein gp120 and confers upon some cell lines the capacity to enhance trans-infection. Using a short hairpin RNA approach, we demonstrate that DC-SIGN is not required for efficient trans-enhancement by DCs. In addition, the DC-SIGN ligand mannan and an anti-DC-SIGN antibody did not inhibit DC-mediated enhancement. HIV-1 particles were internalized and were protected from protease treatment following binding to DCs, but not from binding to DC-SIGN-expressing Raji cells. Thus, DC-SIGN is not required for DC-mediated trans-enhancement of HIV infectivity.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cell Adhesion Molecules / genetics
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Cell Adhesion Molecules / physiology*
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DNA / genetics
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Dendritic Cells / immunology*
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Dendritic Cells / virology*
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Fetal Blood / cytology
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Gene Expression
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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HIV Envelope Protein gp120 / physiology
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HIV Infections / immunology
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HIV Infections / virology
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HIV-1 / immunology*
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HIV-1 / pathogenicity*
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Humans
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In Vitro Techniques
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Infant, Newborn
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Lectins, C-Type / genetics
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Lectins, C-Type / physiology*
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / physiology*
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
Substances
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Cell Adhesion Molecules
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DC-specific ICAM-3 grabbing nonintegrin
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HIV Envelope Protein gp120
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Lectins, C-Type
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Receptors, Cell Surface
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Recombinant Proteins
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Green Fluorescent Proteins
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DNA