Heterologous production of albicidin: a promising approach to overproducing and characterizing this potent inhibitor of DNA gyrase

Antimicrob Agents Chemother. 2007 Apr;51(4):1549-52. doi: 10.1128/AAC.01450-06. Epub 2007 Jan 12.

Abstract

The phytotoxin and polyketide antibiotic albicidin produced by Xanthomonas albilineans is a highly potent DNA gyrase inhibitor. Low yields of albicidin production have slowed studies of its chemical structure. Heterologous expression of albicidin biosynthetic genes in X. axonopodis pv. vesicatoria resulted in a sixfold increase in albicidin production, offering promising strategies for engineering overproduction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / biosynthesis*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Drug Resistance / genetics*
  • Organic Chemicals / metabolism
  • Organic Chemicals / pharmacology
  • Plasmids / genetics*
  • Plasmids / metabolism
  • Topoisomerase II Inhibitors*
  • Xanthomonas / enzymology
  • Xanthomonas / genetics*
  • Xanthomonas / metabolism

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • Organic Chemicals
  • Topoisomerase II Inhibitors
  • albicidin