Abstract
A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Typing Techniques
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Coronavirus / classification*
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Coronavirus / genetics
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Coronavirus / isolation & purification
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Coronavirus 229E, Human / classification
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Coronavirus 229E, Human / genetics
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Coronavirus 229E, Human / isolation & purification
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Coronavirus OC43, Human / classification
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Coronavirus OC43, Human / genetics
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Coronavirus OC43, Human / isolation & purification
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Humans
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Oligonucleotide Array Sequence Analysis / methods*
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RNA, Viral / analysis
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RNA, Viral / genetics
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Severe acute respiratory syndrome-related coronavirus / classification
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Severe acute respiratory syndrome-related coronavirus / genetics
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Severe acute respiratory syndrome-related coronavirus / isolation & purification
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Species Specificity