Comment on "Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake"

Science. 2007 Feb 9;315(5813):766; author reply 766. doi: 10.1126/science.1135047.

Abstract

Zhang et al. (Research Articles, 11 November 2005, p. 996) reported that obestatin, a peptide derived from the ghrelin precursor, activated the orphan G protein-coupled receptor GPR39. However, we found that I125-obestatin does not bind GPR39 and observed no effects of obestatin on GPR39-transfected cells in various functional assays (cyclic adenosine monophosphate production, calcium mobilization, and GPR39 internalization). Our results indicate that obestatin is not the cognate ligand for GPR39.

Publication types

  • Comment

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CHO Cells
  • Calcium / metabolism
  • Cell Membrane / metabolism
  • Colforsin / pharmacology
  • Cricetinae
  • Cricetulus
  • Cyclic AMP / metabolism
  • Ghrelin
  • Humans
  • Ligands
  • Molecular Sequence Data
  • Peptide Hormones / genetics
  • Peptide Hormones / metabolism*
  • Peptide Hormones / pharmacology
  • Pituitary Gland / cytology
  • Pituitary Gland / metabolism
  • Protein Binding
  • Receptors, G-Protein-Coupled / genetics
  • Receptors, G-Protein-Coupled / metabolism*
  • Transfection

Substances

  • GPR39 protein, human
  • Ghrelin
  • Ligands
  • Peptide Hormones
  • Receptors, G-Protein-Coupled
  • obestatin, human
  • Colforsin
  • Cyclic AMP
  • Calcium