High level expression of STAG1/PMEPA1 in an androgen-independent prostate cancer PC3 subclone

Cell Mol Biol Lett. 2007 Sep;12(3):370-7. doi: 10.2478/s11658-007-0009-y. Epub 2007 Feb 17.

Abstract

In this paper, we describe the isolation and characterization of two PC3 subclones. One subclone, mr, showed an epithelial phenotype, the other, M1, showed a sarcomatous morphology. Transplanted into nude mice, mr developed tumors at a dramatically faster rate than M1. Comparing the two subclones, differentially expressed genes were identified, including E-cadherin, IL-8 and STAG1/PMEPA1. These genes were expressed at higher levels in mr than in M1.

MeSH terms

  • Androgens / metabolism*
  • Animals
  • Antigens, CD
  • Cadherins / metabolism
  • Cell Line, Tumor
  • Cell Proliferation
  • Clone Cells
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Interleukin-8 / genetics
  • Interleukin-8 / metabolism
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / pathology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Subcutaneous Tissue / pathology
  • Xenograft Model Antitumor Assays

Substances

  • Androgens
  • Antigens, CD
  • CDH1 protein, human
  • CXCL8 protein, human
  • Cadherins
  • Interleukin-8
  • Nuclear Proteins
  • STAG1 protein, human