Effects of intrauterine inflammation on the developing mouse brain

Brain Res. 2007 May 4:1144:180-5. doi: 10.1016/j.brainres.2007.01.083. Epub 2007 Jan 30.

Abstract

Clinical and experimental evidence indicate that the presence of intrauterine inflammation in pregnancy is not only a cause of preterm birth but is also associated with perinatal brain damage and long-term neurological handicap. In the present study, the neuropathological outcome was investigated in surviving pups in a model of inflammation-induced preterm delivery. C57BL/6 mice were subjected to intrauterine injection of lipopolysaccharide (LPS) or saline, at a time corresponding to 79% of average gestation (gestational day 15). Fetuses that survived after LPS administration were sacrificed on postnatal day 14 (PND 14). At PND 14, the brain weight of LPS-exposed pups was significantly lower than that of saline-exposed. A high proportion of LPS-exposed brains were found affected and exhibited hypomyelination, enlarged ventricles, and in some cortical gray matter lesions were evident. None of these pathologies were detected in sham-treated animals.

Conclusions: Intrauterine inflammation impaired brain development and various brain lesions were produced in both the white and gray matter after intrauterine LPS administration in mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Body Weight / drug effects
  • Body Weight / physiology
  • Brain / growth & development*
  • Brain / pathology*
  • Female
  • Inflammation / mortality
  • Inflammation / pathology*
  • Inflammation / physiopathology*
  • Lipopolysaccharides*
  • Mice
  • Mice, Inbred C57BL
  • Myelin Basic Protein / metabolism
  • Organ Size / drug effects
  • Organ Size / physiology
  • Pregnancy
  • Prenatal Exposure Delayed Effects* / chemically induced
  • Prenatal Exposure Delayed Effects* / pathology
  • Prenatal Exposure Delayed Effects* / physiopathology

Substances

  • Lipopolysaccharides
  • Myelin Basic Protein