Matrix metalloproteinases (MMPs) are enzymes that play a central role in periodontal ligament (PDL) space remodelling during orthodontic tooth movement. It has previously been shown that messenger RNA levels of MMP-13 increase significantly following the application of orthodontic forces. The aim of the present study was to examine immunolocalisation of MMP-13 and to evaluate if this collagenase is time-dependently and differentially detected within the PDL following the application of orthodontic forces to create areas of compression and tension. This was achieved by placing elastic bands between the maxillary first and second molars of 16 male Sprague-Dawley rats (each weighing 120-200g) for 12 and 24h. The molar-bearing segments were dissected and processed for histological and immunohistochemical examination. Binding of a monoclonal antibody was used to evaluate MMP-13 localization using an indirect streptavidin/biotin immunperoxidase technique. MMP-13 was found to be inducible at the protein level by the application of forces. The PDL and osteoblast-lineage cells showed a time-dependent increase in immunolabelling of MMP-13. Immunolabelling of MMP-13 was detected initially on the compression side, and then on both the compression and the tension sides. Since this increase in MMP-13 immunolabelling occurred very early following the application of an orthodontic force in both PDL and alveolar bone, this would indicate that MMP-13 might play an important role during tooth movement.