Using an indicator displacement assay to monitor glucose oxidase activity in blood serum

Org Lett. 2007 Apr 26;9(9):1627-9. doi: 10.1021/ol070280o. Epub 2007 Mar 29.

Abstract

[diagram: see text] Using a boronic acid receptor that was previously found to have high affinity for gluconic acid, we created a colorimetric indicator displacement assay (IDA) that can report the concentration of the product of glucose oxidase (GOx) catalyzed glucose oxidation. The color change obtained directly reflects the concentration of glucose. Our sensing ensemble was then successfully applied to determine the glucose concentration in human serum, which offers a facile, colorimetric, sensitive, and accurate glucose test.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Glucose / analysis
  • Blood Glucose / metabolism
  • Catalysis
  • Colorimetry / methods*
  • Gluconates / chemistry
  • Gluconates / metabolism
  • Glucose Oxidase / blood*
  • Glucose Oxidase / metabolism*
  • Humans
  • Molecular Structure
  • Oxidation-Reduction
  • Titrimetry

Substances

  • Blood Glucose
  • Gluconates
  • Glucose Oxidase
  • gluconic acid