Effects of engrafted neural stem cells derived from GFP transgenic mice in Parkinson's diseases rats

Neurosci Lett. 2007 May 23;419(1):49-54. doi: 10.1016/j.neulet.2007.03.046. Epub 2007 Mar 25.

Abstract

This study evaluated the therapeutic effect of neural stem cells (NSCs) transplanted into Parkinson's disease (PD) rats. NSCs were identified in vitro, then engrafted into the striatum of the PD rats. The rotational behavior was evaluated 1, 2, 4 and 6 weeks. A significant rotational behavior improvement was observed in PD rats subjected to cell transplantation. Transplanted NSCs not only express Nerve growth factor and Neurotrophin-3 in vitro, but also survive and partly differentiate into tyrosine hydroxylase (TH) positive cells in vivo. The results show that NSCs could be effective for PD treatment and the mechanisms might involve the neurotrophin expression and the neural differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Antiparkinson Agents / pharmacology
  • Apomorphine / pharmacology
  • Behavior, Animal
  • Disease Models, Animal
  • Glial Fibrillary Acidic Protein / metabolism
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism*
  • Mice
  • Mice, Transgenic
  • Neurons / physiology*
  • Oxidopamine / toxicity
  • Parkinson Disease / drug therapy
  • Parkinson Disease / etiology
  • Parkinson Disease / surgery*
  • Rats
  • Rats, Sprague-Dawley
  • Stem Cell Transplantation / methods*
  • Stem Cells / physiology*
  • Stereotyped Behavior / drug effects
  • Sympatholytics / toxicity
  • Time Factors
  • Transplantation, Heterologous
  • Tyrosine 3-Monooxygenase / metabolism

Substances

  • Antiparkinson Agents
  • Glial Fibrillary Acidic Protein
  • Sympatholytics
  • Green Fluorescent Proteins
  • Oxidopamine
  • Tyrosine 3-Monooxygenase
  • Apomorphine