Mast cell distribution, activation, and phenotype in xanthoma

J Am Acad Dermatol. 2007 Jun;56(6):1006-12. doi: 10.1016/j.jaad.2006.05.071.

Abstract

Background: Activated mast cells enhance the uptake of mast cell-derived proteoglycan-low-density lipoprotein complexes by macrophages.

Objective: We sought to investigate mast cell contribution to the pathogenesis of xanthoma.

Methods: Twenty cases of xanthelasma palpebrarum and 6 cases of tuberous xanthoma lesions were analyzed using immunohistochemical staining.

Results: Xanthelasma lesions contained up to 5-fold more tryptase-stained mast cells than tuberous xanthoma lesions. Tuberous xanthoma lesions especially showed extensive staining of tryptase around mast cells and within some macrophages and foam cells. More than 99% of mast cells in xanthelasma lesions contained both tryptase and chymase. Approximately 60% of mast cells represented only tryptase in tuberous xanthoma lesions where the ratio of macrophages to tryptase-stained mast cells was extremely high (15:1) as compared with xanthelasma lesions (2:1).

Limitations: A change in mast cell phenotype has not been necessarily proven.

Conclusion: Mast cells are activated under the microenvironment in which macrophages predominate rather than mast cells, which thus reflects the clinical phenotypes of xanthoma lesions.

MeSH terms

  • Aged
  • Cell Differentiation / physiology
  • Chymases / metabolism
  • Coloring Agents
  • Female
  • Foam Cells / metabolism
  • Humans
  • Immunohistochemistry
  • Macrophages / metabolism*
  • Male
  • Mast Cells / metabolism*
  • Middle Aged
  • Phenotype
  • Skin / metabolism
  • Tolonium Chloride
  • Tryptases / metabolism
  • Xanthomatosis / metabolism*

Substances

  • Coloring Agents
  • Tolonium Chloride
  • Chymases
  • Tryptases