Stable transcriptional status in the apoptotic erythroid genome

Biochem Biophys Res Commun. 2007 Aug 3;359(3):556-62. doi: 10.1016/j.bbrc.2007.05.168. Epub 2007 Jun 4.

Abstract

When a cell is destined for apoptosis, will its genome reprogram its transcriptional machinery to overcome the life-threatening challenge? To address this issue, we performed a genome-wide transcriptome analysis in EPO (erythropoietin) deprivation-induced apoptotic erythroid cells using the SAGE method. The results show that the transcript contents for the majority of the genes remain unchanged in the apoptotic cells, including the apoptotic genes and the heat shock genes. Of the small number of genes with an altered expression, they are mainly associated with cellular structure. Our study reveals that there is no genetic reprogramming for the transcriptional machinery in the apoptotic genome. Apoptosis, as defined by programmed cell death, is not a crisis but a peaceful physiological process.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis* / drug effects
  • Cells, Cultured
  • Erythroid Cells / cytology*
  • Erythroid Cells / drug effects
  • Erythroid Cells / metabolism*
  • Erythropoietin / pharmacology
  • Gene Expression Regulation
  • Genome, Human / genetics*
  • Heat-Shock Proteins / genetics
  • Humans
  • RNA, Messenger / genetics
  • Stem Cells / cytology
  • Stem Cells / drug effects
  • Stem Cells / metabolism
  • Time Factors
  • Transcription, Genetic / genetics*

Substances

  • Heat-Shock Proteins
  • RNA, Messenger
  • Erythropoietin