Difficulties in classifying a/g recombinants: methodological problems or genetic variability?

AIDS Res Hum Retroviruses. 2007 Jun;23(6):840-6. doi: 10.1089/aid.2006.0205.

Abstract

Thirty pol gene plasma-derived sequences clustering with the circulating recombinant form (CRF) 02_AG (IbNG) (bootstrap 100%) were evaluated to analyze the genomic composition. Subtype assignment was also phylogenetically confirmed by C2-V3 region analysis for 18/21 sequences evaluated. Thereafter, we compared the genomic recombination of the CRF02_AG/IbNG prototype as predicted by bootscanning and Jumping HMMER software (jpHMM) to that of our strains. With these methods, 27% and 50%, respectively, of our clinical sequences demonstrated the same pol structure as the prototype CRF02_A/G-IbNG. However, in subtrees built for each segment predicted by jpHMM (with a bootstrap value of more than 75%), all fragments clustered with IbNG and were distinct from A and G clades. Overall, our sequences resulted in true members of CRF02_AG-IbNG, which, however, appeared to be a subtype phylogenetically separate from A or G, at least with regard to the pol gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Black People / ethnology
  • Child, Preschool
  • Emigration and Immigration
  • Female
  • Genes, env / genetics
  • Genes, pol / genetics*
  • HIV / classification
  • HIV / genetics*
  • HIV Infections / epidemiology
  • HIV Infections / genetics*
  • Humans
  • Italy / epidemiology
  • Male
  • Molecular Sequence Data
  • Phylogeny
  • Reassortant Viruses / classification
  • Reassortant Viruses / genetics*
  • Sexual Behavior

Associated data

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