Abstract
The Pseudomonas aeruginosa polysaccharide synthesis locus (psl) is predicted to encode an exopolysaccharide which is critical for biofilm formation. Here we used chemical composition analyses and mannose- or galactose-specific lectin staining, followed by confocal laser scanning microscopy and electron microscopy, to show that Psl is a galactose-rich and mannose-rich exopolysaccharide.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Galactose / analysis*
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Galactose / chemistry
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Mannose / analysis*
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Mannose / chemistry
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Polysaccharides, Bacterial / biosynthesis
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Polysaccharides, Bacterial / chemistry*
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Polysaccharides, Bacterial / genetics
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Pseudomonas aeruginosa / chemistry*
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Pseudomonas aeruginosa / genetics
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Pseudomonas aeruginosa / metabolism
Substances
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Polysaccharides, Bacterial
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exopolysaccharide, Pseudomonas
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Mannose
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Galactose