Cell force microscopy on elastic layers of finite thickness

Biophys J. 2007 Nov 1;93(9):3314-23. doi: 10.1529/biophysj.107.111328. Epub 2007 Jul 27.

Abstract

Forces applied by cells to substrates can be measured using soft substrates with embedded displacement markers. Traction forces are retrieved from microscopic images by determining the displacements of these markers and fitting the generating forces. Here we show that using elastic films of 5-10-microm thickness one can improve the spatial resolution of the technique. To this end we derived explicit equations for the mechanical response of an elastic layer of finite thickness to point forces. Moreover, these equations allow highly accurate force measurements on eukaryotic cells on films where finite thickness effects are relevant (below approximately 60 microm).

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Elasticity
  • Fibroblasts / ultrastructure
  • Microscopy, Atomic Force*
  • Myocytes, Cardiac / ultrastructure
  • Rats
  • Rats, Wistar
  • Silicon*

Substances

  • Silicon