Cryptococcus neoformans typing by PCR fingerprinting using (GACA)4 primers based on C. neoformans genome project data

Abstract

Four (GACA)(4) PCR fingerprinting sequences, used as markers to identify serotypes A and D and AD hybrids, were retrieved in four Cryptococcus neoformans genome databases. Their locations, both in serotype A and D genomes, were confirmed by chromosomal hybridization with specific probes. Two sequences were recognized to code for hypothetical functional proteins.