Abstract
Skeletal muscles are frequently analyzed for composition of phenotypically distinct myofibers, as a functional determinant. We describe an improved myofiber phenotyping procedure, involving cryosection co-incubation with fluorophore-labeled myosin heavy-chain (MyHC)-isoform-specific antibodies. This technique identifies multiple fiber "types" on a single section, thereby reducing reagents and processing, and offers side-by-side comparison of samples from multiple species including mice. These advances are valuable for studying the physiological attributes of skeletal muscle in health and disease.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies* / chemistry
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Antibody Specificity
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Dogs
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Fluorescent Antibody Technique / methods*
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Fluorescent Dyes
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Hybridomas
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Immunophenotyping / methods*
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Macaca fascicularis
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Mice
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Mice, Inbred C57BL
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Muscle Fibers, Skeletal / chemistry
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Muscle Fibers, Skeletal / classification*
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Muscle Fibers, Skeletal / cytology*
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Muscle, Skeletal / chemistry
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Muscle, Skeletal / cytology*
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Myosin Heavy Chains / analysis*
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Myosin Heavy Chains / immunology
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Rats
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Rats, Sprague-Dawley
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Reproducibility of Results
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Sensitivity and Specificity
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Species Specificity
Substances
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Antibodies
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Fluorescent Dyes
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Myosin Heavy Chains