Despite improved diagnostic methods, antimicrobial agents and more sophisticated echocardiographic studies, the incidence of infective endocarditis remains high, especially in a uremic and end stage renal disease (ESRD) population. Culture negative endocarditis (CNE) patients with normal renal function have increased morbidity and mortality, but clinical features and outcome in hemodialysis patients remains unclear. This study reported five survival cases of CNE in hemodialysis patients. The prognosis of all dialysis-related CNE patients enrolled in this study was excellent.