Abstract
5-Arylidene-3-hydroxyalkyl-2-phenylimino-4-thiazolidinones (7,8) were synthesized and evaluated for their antidegenerative activity on human chondrocyte cultures stimulated by IL-1beta. This in vitro model has proven to be a useful experimental model to reproduce the mechanisms involved in arthritic diseases. The cell viability, the amount of GAGs, the production of NO and PGE(2) and the inhibition of MMP-3 were measured. Several thiazolidinones 7 and 8 exhibited the ability to block the production or action of the degenerative factors induced by IL-1beta.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Inflammatory Agents, Non-Steroidal / chemical synthesis*
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Arthritis / drug therapy
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Cell Survival / drug effects
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Cells, Cultured
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Chondrocytes / drug effects*
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Chondrocytes / immunology
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Drug Evaluation, Preclinical
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Humans
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Imines / chemical synthesis*
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Imines / pharmacology
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Indomethacin / pharmacology
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Interleukin-1beta / pharmacology
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Models, Biological*
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Reference Standards
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Thiazolidines / chemical synthesis*
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Thiazolidines / pharmacology*
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Imines
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Interleukin-1beta
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Thiazolidines
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Indomethacin