Molecular typing of a suspected cluster of Nocardia farcinica infections by use of randomly amplified polymorphic DNA, pulsed-field gel electrophoresis, and amplified fragment length polymorphism analyses

J S Kalpoe; K E Templeton; A M Horrevorts; H P Endtz; E J Kuijper; A T Bernards; C H W Klaassen

doi:10.1128/JCM.00932-07

Molecular typing of a suspected cluster of Nocardia farcinica infections by use of randomly amplified polymorphic DNA, pulsed-field gel electrophoresis, and amplified fragment length polymorphism analyses

J Clin Microbiol. 2007 Dec;45(12):4048-50. doi: 10.1128/JCM.00932-07. Epub 2007 Oct 3.

Authors

J S Kalpoe¹, K E Templeton, A M Horrevorts, H P Endtz, E J Kuijper, A T Bernards, C H W Klaassen

Affiliation

¹ Department of Medical Microbiology, E4-P, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands. J.S.Kalpoe@lumc.nl

Abstract

Randomly amplified polymorphic DNA (RAPD), pulsed-field gelelectrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) analyses were used to investigate a possible outbreak of Nocardia farcinica. RAPD and PFGE analyses yielded irreproducible and unsatisfactory results, respectively. AFLP analysis seem to be a promising and welcome addition for molecular analysis of Nocardia isolates.

MeSH terms

Aged
Cluster Analysis
DNA Fingerprinting*
DNA, Bacterial / genetics*
Electrophoresis, Gel, Pulsed-Field
Female
Humans
Male
Middle Aged
Molecular Epidemiology / methods
Nocardia / classification*
Nocardia / genetics*
Nocardia / isolation & purification
Nocardia Infections / epidemiology*
Nocardia Infections / microbiology*
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique

Substances

DNA, Bacterial