In calcareous salt-affected soils, iron availability to plants is subjected to the effects of both sodium and bicarbonate ions. Our aim was to study interactive effects of salinity and iron deficiency on iron acquisition and root acidification induced by iron deficiency in Medicago ciliaris L., a species commonly found in saline ecosystems. Four treatments were used: C, control treatment, complete medium (CM) containing 30 microM Fe; S, salt treatment, CM with 75 mM NaCl; D, deficient treatment, CM containing only 1 microM Fe; DS, interactive treatment, CM containing 1 microM Fe with 75 mM NaCl. Our study showed that plant growth and chlorophyll content were much more affected by the interactive treatment than by iron deficiency or by the salt treatment, indicating an additive effect of these constraints in DS plants. These results could be partially explained by Na accumulation in shoots as well as a limitation of nutrient uptake such as Fe and K under salt stress, under iron deficiency, and especially under their combined effect. The study also showed that root acidification was deeply diminished when iron deficiency was associated with salinity. This probably explained the decrease of Fe uptake and suggested that root proton pump activity would be inhibited by salinity.