Group A rotavirus-associated diarrhea in children seeking treatment in Indonesia

J Clin Virol. 2007 Dec;40(4):289-94. doi: 10.1016/j.jcv.2007.09.005. Epub 2007 Oct 30.

Abstract

Background: Globally, group A rotavirus causes significant morbidity and mortality among children. Limited data exist on the epidemiology of rotavirus disease among Indonesian children.

Objectives: We describe the epidemiology of rotavirus-associated diarrhea among Indonesian children <6 years of age, including clinical symptoms and genotypes.

Study design: We conducted a hospital-based, case series study at four referral hospitals between February 2004 and February 2005 among children with diarrhea. Rotavirus positivity was defined by a positive result from either EIA or RT-PCR. A semi-nested RT-PCR was used to determine specific rotavirus genotypes.

Results: 1660 stools were tested for pathogens. The overall rotavirus prevalence was 45.5%. Children with rotavirus-associated diarrhea were significantly younger (p<0.0001) and more likely to be hospitalized (81.3% versus 72.2%; p<0.0001). Symptoms associated with rotavirus included, vomiting, fever, nausea, fatigue and dehydration, while bloody stool was significantly less common with rotavirus-associated diarrhea.

Conclusion: Rotavirus was an important contributor of morbidity to our study sample. Rotavirus genotyping demonstrated a temporal shift from G1-G4 to G9, but this was highly associated with the P[8] gene, suggesting that a multivalent rotavirus vaccine, incorporating G9 P[8] antigen, may reduce the burden of diarrheal illnesses among Indonesian children.

MeSH terms

  • Child, Preschool
  • Dysentery / epidemiology
  • Dysentery / virology*
  • Feces / virology
  • Female
  • Genotype
  • Humans
  • Immunoenzyme Techniques / methods
  • Indonesia / epidemiology
  • Infant
  • Infant, Newborn
  • Male
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Rotavirus / genetics
  • Rotavirus / isolation & purification*
  • Rotavirus Infections / epidemiology
  • Rotavirus Infections / virology*