Comparison of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR assay to culture by use of BBL CHROMagar MRSA for detection of MRSA in nasal surveillance cultures from an at-risk community population

J Clin Microbiol. 2008 Feb;46(2):743-6. doi: 10.1128/JCM.02071-07. Epub 2007 Dec 5.

Abstract

We compared the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR assay to culture with BBL CHROMagar MRSA for nasal surveillance among 602 arrestees from the Baltimore City Jail. The sensitivity and specificity were 88.5% and 91.0%, respectively, and after secondary analysis using enrichment broth, they were 89.0% and 91.7%, respectively. Twenty-three of 42 false-positive PCR lysates contained methicillin-susceptible S. aureus.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Bacteriological Techniques / methods*
  • Baltimore
  • Carrier State / diagnosis*
  • Carrier State / microbiology
  • Culture Media / chemistry
  • False Positive Reactions
  • Humans
  • Male
  • Methicillin Resistance*
  • Middle Aged
  • Nose / microbiology*
  • Polymerase Chain Reaction / methods
  • Prisons
  • Sensitivity and Specificity
  • Staphylococcal Infections / diagnosis*
  • Staphylococcal Infections / microbiology
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / isolation & purification*

Substances

  • Culture Media