Pyrene-functionalized 2'-amino-alpha-L-LNA as potential diagnostic probes

T Santhosh Kumar; Jesper Wengel; Patrick J Hrdlicka

doi:10.1080/15257770701538908

Pyrene-functionalized 2'-amino-alpha-L-LNA as potential diagnostic probes

Nucleosides Nucleotides Nucleic Acids. 2007;26(10-12):1407-9. doi: 10.1080/15257770701538908.

Authors

T Santhosh Kumar¹, Jesper Wengel, Patrick J Hrdlicka

Affiliation

¹ Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, 5230 Odense M, Denmark. skt@chem.sdu.dk

PMID: 18066793
DOI: 10.1080/15257770701538908

Abstract

Oligodeoxyribonucleotides (ONs) containing two incorporations of 2'-N-(pyren-1-yl)acetyl-2'-amino-alpha-L-LNA monomer Y are sensitive probes for detection of single nucleotide polymorphisms (SNP) in DNA. In addition, the ability of ONs containing pyrene-functionalized 2'-amino-alpha-L-LNA monomers (W-Z) to stabilize duplexes with an abasic site is demonstrated.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Fluorescent Dyes / chemistry*
Humans
Oligonucleotides / chemistry*
Polymorphism, Single Nucleotide*
Sequence Analysis, DNA*
Thymidine Monophosphate / analogs & derivatives*
Thymidine Monophosphate / chemistry

Substances

2'-N-(pyren-1-yl)acetyl-2'-amino-alpha-L-LNA
Fluorescent Dyes
Oligonucleotides
Thymidine Monophosphate