The aim was to assess the prevalent defense mechanisms in a sample of obese subjects; since specific defensive styles may interfere with the management of stressors and emotions, they may also influence the onset, the severity, and the maintenance of obesity. 70 obese subjects and 70 healthy normal-weight volunteers were assessed using the Defense Mechanisms Inventory -- DMI. Significant differences between groups have emerged at Turning Against Object (t=-5.30; p<0.0001), Projection (t=-5.55; p<0.0001), Turning Against Self (t=-4.87; p<0.0001) and Reversal (t=-3.61; p<0.0001) variables. Within the obese group, significant differences have been found at Turning Against Object (U=264; p=.001) and Projection (U=359; p=.042) scales, both higher in males. No significant differences on DMI scores in relation to the severity of obesity have been observed. An inadequate defensive structure might represent a vulnerability to emotional states and stressful life events. The assessment of defense mechanisms may provide a valid tool for long-term treatments of obesity.