DNA microarray analysis of central carbohydrate metabolism: glycolytic/gluconeogenic carbon switch in the hyperthermophilic crenarchaeum Thermoproteus tenax

J Bacteriol. 2008 Mar;190(6):2231-8. doi: 10.1128/JB.01524-07. Epub 2008 Jan 4.

Abstract

In order to unravel the role of regulation on transcript level in central carbohydrate metabolism (CCM) of Thermoproteus tenax, a focused DNA microarray was constructed by using 85 open reading frames involved in CCM. A transcriptional analysis comparing heterotrophic growth on glucose versus autotrophic growth on CO2-H2 was performed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Metabolism*
  • Gene Expression Regulation, Archaeal / drug effects
  • Gluconeogenesis
  • Glucose / metabolism
  • Glucose / pharmacology
  • Glycolysis
  • Hot Temperature
  • Oligonucleotide Array Sequence Analysis / methods*
  • Open Reading Frames / genetics
  • Thermoproteus / genetics*
  • Thermoproteus / growth & development
  • Thermoproteus / metabolism*
  • Transcription, Genetic / drug effects

Substances

  • Glucose