Abstract
We developed a real-time reverse transcription--PCR that detected 1,164 copies/mL of Crimean-Congo hemorrhagic fever virus per milliliter of serum at 95% probability (probit analysis) and was 100% concordant with nested PCR on 63 samples from 31 patients with confirmed infection. Infected patients who died appeared to have higher viral loads; low viral loads correlated with IgG detection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Genetic Variation*
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Hemorrhagic Fever Virus, Crimean-Congo / classification
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Hemorrhagic Fever Virus, Crimean-Congo / genetics
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Hemorrhagic Fever Virus, Crimean-Congo / isolation & purification*
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Hemorrhagic Fever, Crimean / blood
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Hemorrhagic Fever, Crimean / diagnosis*
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Hemorrhagic Fever, Crimean / mortality
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Humans
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Phylogeny
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Reproducibility of Results
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Reverse Transcriptase Polymerase Chain Reaction / standards*
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Sensitivity and Specificity
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Species Specificity
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Viral Load*