Establishment of idiotype bearing B-lymphocyte clones from a patient with monoclonal gammopathy

Blood. 1991 Nov 15;78(10):2642-9.

Abstract

B-lymphocyte clones bearing an idiotypic Ig structure, which could also be found on the serum M-component, have been isolated from peripheral blood of a patient with monoclonal gammopathy (MG) using an immune-rosetting technique and limiting dilution. The majority of the cells had the surface phenotype id+/CD19+/CD20+/CD21+ or id+/CD19+/CD20+/CD21-. Some of the clones originated from spontaneous outgrowth of lymphocytes and some were derived from in vitro Epstein-Barr virus-infected lymphocytes. Three of the B-lymphocyte clones and the patient's bone marrow plasma cells appeared to have clonal identity as shown by the same Ig heavy chain gene rearrangements in Southern blot analysis using a JH probe. The study further supports the notion that the peripheral blood of patients with MG contains B lymphocytes that belong to the tumor clone.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Antigens, CD / analysis
  • B-Lymphocytes / immunology*
  • Blotting, Southern
  • Cell Line
  • Clone Cells
  • DNA / blood
  • DNA / genetics
  • DNA / isolation & purification
  • Female
  • Fluorescent Antibody Technique
  • Gene Rearrangement
  • Genes, Immunoglobulin
  • Humans
  • Immunoglobulin Fab Fragments / analysis
  • Immunoglobulin G / analysis*
  • Immunoglobulin G / isolation & purification
  • Immunoglobulin Heavy Chains / genetics*
  • Immunoglobulin Idiotypes / analysis*
  • Isoelectric Focusing
  • Middle Aged
  • Paraproteinemias / genetics
  • Paraproteinemias / immunology*
  • Phenotype
  • Rosette Formation

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Immunoglobulin Fab Fragments
  • Immunoglobulin G
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Idiotypes
  • DNA