Abstract
Purification of the obligate intracellular bacterium Coxiella burnetii requires physical disruption of infected cells. Here we describe a gentle and safe digitonin lysis procedure to release C. burnetii from infected cells. The purity, yield, and infectivity of digitonin-prepped organisms are comparable to that of organisms purified using cell lysis by sonication.
Publication types
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Evaluation Study
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Research Support, N.I.H., Intramural
MeSH terms
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Animals
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Bacteriological Techniques / methods*
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Cell Membrane / drug effects
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Chlorocebus aethiops
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Coxiella burnetii / cytology
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Coxiella burnetii / isolation & purification*
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Coxiella burnetii / ultrastructure
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Digitonin / metabolism*
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Microscopy
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Microscopy, Electron, Transmission
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Vero Cells