Abstract
The microbiological study identifies the bacterial spectrum after surgery, in acute, delayed-onset, or chronic endophthalmitis. DNA amplification of eubacterium-specific sequences in DNA extracted from ocular samples is a new tool for the etiological diagnosis of endophthalmitis. The most successful way to identify bacteria in endophthalmitis is the association of conventional cultures and panbacterial PCR on vitreous samples. Both techniques are complementary. The efficacy of these new molecular techniques should modify our future therapeutic strategies.
MeSH terms
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Acute Disease
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Aqueous Humor / microbiology
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Bacterial Infections / diagnosis*
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Bacterial Infections / drug therapy
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Bacterial Infections / microbiology
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Bacteriological Techniques
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Endophthalmitis / diagnosis*
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Endophthalmitis / drug therapy
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Endophthalmitis / microbiology
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Eubacterium / genetics
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Eubacterium / isolation & purification
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Eye Injuries, Penetrating / complications*
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Eye Injuries, Penetrating / microbiology
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Gram-Positive Bacterial Infections / diagnosis
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Gram-Positive Bacterial Infections / drug therapy
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Gram-Positive Bacterial Infections / microbiology
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Humans
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Microbial Sensitivity Tests
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Polymerase Chain Reaction
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Predictive Value of Tests
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Sequence Analysis, DNA
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Staphylococcal Infections / diagnosis
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Staphylococcal Infections / microbiology
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Streptococcal Infections / diagnosis
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Streptococcal Infections / microbiology
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Surgical Wound Infection / diagnosis*
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Surgical Wound Infection / drug therapy
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Surgical Wound Infection / microbiology
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Vitrectomy
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Vitreous Body / microbiology