Noninvasive ultrasound elasticity imaging (UEI) of Crohn's disease: animal model

Ultrasound Med Biol. 2008 Jun;34(6):902-12. doi: 10.1016/j.ultrasmedbio.2007.11.020. Epub 2008 Feb 21.

Abstract

Inflammation occurs in episodic flares in Crohn's disease, which are part of the waxing and waning course of the disease. Healing between flares allows the intestine to reconstitute its epithelium, but this healing results in the deposition of fibrotic scar tissue as part of the healing process. Repeated cycles of flares and healing often lead to clinically significant fibrosis and stenosis of the intestine. Patients are treated empirically with steroids, with their many side effects, in the hope that they will respond. Many patients would be better treated with surgery if we could identify which patients truly have intestinal fibrosis. Ultrasound elasticity imaging (UEI) offers the potential to radically improve the diagnosis and management of local tissue elastic property, particularly intestinal fibrosis. This method allows complete characterization of local intestine tissue with high spatial resolution. The feasibility of UEI on Crohn's disease is demonstrated by directly applying this technique to an animal model of inflammatory bowel disease (IBD). Five female Lewis rats (150-180g) were prepared with phosphate buffered solution (PBS) as a control group and six were prepared with repeated intrarectal administration of trinitrobenzenesulfonic acid (TNBS) as a disease group. Preliminary strain measurements differentiate the diseased colons from the normal colons (p < 0.0002) and compared well with direct mechanical measurements and histology (p < 0.0005). UEI provides a simple and accurate assessment of local severity of fibrosis. The preliminary results on an animal model also suggest the feasibility of translating this imaging technique directly to human subjects for both diagnosis and monitoring.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Colon / diagnostic imaging*
  • Colon / pathology
  • Crohn Disease / diagnostic imaging*
  • Crohn Disease / pathology
  • Disease Models, Animal
  • Elasticity
  • Elasticity Imaging Techniques / methods*
  • Female
  • Fibrosis
  • Image Interpretation, Computer-Assisted*
  • In Vitro Techniques
  • Rats
  • Rats, Inbred Lew
  • Sensitivity and Specificity
  • Stress, Mechanical
  • Trinitrobenzenesulfonic Acid

Substances

  • Trinitrobenzenesulfonic Acid