IgD receptors on murine T-helper cells bind to Fd and Fc regions of immunoglobulin D

Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9233-7. doi: 10.1073/pnas.88.20.9233.

Abstract

Receptors for immunoglobulins on animal cells invariably show specificity for Fc regions of the protein and are hence called Fc receptors. The present study shows that immunoglobulin D receptors present an exception to this rule. Binding of IgD-coated erythrocytes to murine IgD-receptor-bearing T-helper cells is competitively inhibited by IgD, by its Fab delta fragments, and by deletion mutants of IgD lacking (i) the first constant domain of the delta heavy chain (KWD1), (ii) that region plus the delta heavy-chain-hinge region (KWD6), or (iii) the third constant domain of the delta heavy chain (Gen. 24). KWD1, Gen. 24, or KWD6 mutants bind to T-helper cells bearing receptors for IgD independently of each other. Furthermore, Gen. 24 and KWD6 mutants also competitively inhibit binding of each other in cross-blocking experiments. These results show that the IgD receptors binds to the Fd delta and the Fc delta and cannot readily be explained by sequence homology between the two parts of the IgD molecule.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Immunoglobulin Constant Regions / immunology
  • Immunoglobulin Constant Regions / metabolism*
  • Immunoglobulin D / metabolism*
  • Immunoglobulin Heavy Chains / immunology
  • Immunoglobulin Heavy Chains / metabolism*
  • Immunoglobulin delta-Chains / immunology
  • Immunoglobulin delta-Chains / metabolism*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Peptides / chemical synthesis
  • Peptides / immunology
  • Receptors, Fc*
  • Receptors, Immunologic / metabolism*
  • Rosette Formation
  • Spleen / immunology
  • Spleen / metabolism*
  • T-Lymphocytes / immunology*
  • T-Lymphocytes, Helper-Inducer / immunology*

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin Constant Regions
  • Immunoglobulin D
  • Immunoglobulin Heavy Chains
  • Immunoglobulin delta-Chains
  • Peptides
  • Receptors, Fc
  • Receptors, Immunologic
  • immunoglobulin D receptor