Abstract
Rates of detection of rotavirus were compared by diagnostic assay and specimen type. For bulk stools, rates of detection by reverse transcriptase PCR (RT-PCR) and enzyme immunoassay (EIA) were similar, but 18% of healthy controls tested positive by RT-PCR. Testing of bulk stools by EIA appears to be optimum for rotavirus surveillance.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Child, Preschool
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Feces / virology
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Humans
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Immunoenzyme Techniques / methods
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Infant
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Infant, Newborn
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Mass Screening / methods*
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Rotavirus / isolation & purification*
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Rotavirus Infections / diagnosis*
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Sensitivity and Specificity
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Virology / methods*