IL-15 as a mediator of CD4+ help for CD8+ T cell longevity and avoidance of TRAIL-mediated apoptosis

Proc Natl Acad Sci U S A. 2008 Apr 1;105(13):5201-6. doi: 10.1073/pnas.0801003105. Epub 2008 Mar 24.

Abstract

CD4+ helper T cells contribute to the induction and maintenance of antigen-specific CD8+ T cells. Their absence results in short-lived antigen-specific CD8+ T cells and defective secondary CD8+ T cell responses because of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis. Here, we show that IL-15 codelivered with vaccines can overcome CD4+ T cell deficiency for promoting longevity of antigen-specific CD8+ T cells and avoidance of TRAIL-mediated apoptosis. In both priming and secondary responses, IL-15 down-regulates proapoptotic Bax, an intermediate in TRAIL-mediated apoptosis, and increases anti-apoptotic Bcl-X(L) in CD8+ T cells. Thus, IL-15 is sufficient to mimic CD4+ T cell help. Antigen-specific CD4+ T cells induce dendritic cells (DCs) to produce IL-15. IL-15 is also necessary for optimal help, because helper cells do not deliver effective help through IL-15-/- DCs. Therefore, IL-15 codelivered with vaccines can overcome CD4+ helper T cell deficiency for induction of functionally efficient CD8+ T cells and maintenance of CD8+ cytotoxic T lymphocytes (CTLs), and IL-15 is probably one of the natural mediators of help. These findings suggest new vaccine strategies against infections and cancers, especially in individuals with CD4-deficiency.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Antigen-Presenting Cells / immunology
  • Apoptosis / immunology*
  • CD8-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / metabolism*
  • Caspase 3 / metabolism
  • Cell Line
  • Cell Membrane / immunology
  • Cell Membrane / metabolism
  • Dendritic Cells / immunology
  • Dendritic Cells / metabolism
  • Down-Regulation
  • Female
  • Immunologic Memory / immunology
  • Interleukin-15 / deficiency
  • Interleukin-15 / genetics
  • Interleukin-15 / immunology*
  • Interleukin-15 / metabolism
  • Mice
  • Mice, Knockout
  • Neoplasms / immunology
  • Neoplasms / pathology
  • Protein Binding
  • T-Lymphocytes, Helper-Inducer / immunology*
  • TNF-Related Apoptosis-Inducing Ligand / metabolism*
  • Up-Regulation
  • Vaccines / immunology
  • bcl-2-Associated X Protein / metabolism

Substances

  • Interleukin-15
  • TNF-Related Apoptosis-Inducing Ligand
  • Vaccines
  • bcl-2-Associated X Protein
  • Caspase 3