Subtle genomic alterations and genomic instability revealed in diploid cancer cell lines

Xueying Mao; Bryan D Young; Tracy Chaplin; Janet Shipley; Yong-Jie Lu

doi:10.1016/j.canlet.2008.03.003

Subtle genomic alterations and genomic instability revealed in diploid cancer cell lines

Cancer Lett. 2008 Aug 18;267(1):49-54. doi: 10.1016/j.canlet.2008.03.003. Epub 2008 Apr 14.

Authors

Xueying Mao¹, Bryan D Young, Tracy Chaplin, Janet Shipley, Yong-Jie Lu

Affiliation

¹ Centre of Medical Oncology, Institute of Cancer, Barts and London School of Medicine and Dentistry, Queen Mary, University of London, Charterhouse Square, London EC1M 6BQ, UK.

PMID: 18407410
DOI: 10.1016/j.canlet.2008.03.003

Abstract

To investigate if genomic instability exists in tumors with cytogenetically normal karyotypes, we analyzed four diploid cancer cell lines A204, CAL51, CH1 and SK-UT-1B. We detected subtle genomic changes in all four cell lines. More of these alterations were found in A204 and CH1 than in the microsatellite unstable lines CAL51 and SK-UT-1B. The number of de novo, non-clonal chromosome rearrangements was also significantly higher in CH1 than CAL51 and SK-UT-1B (p=0.001). This study reveals multiple genomic abnormalities in tumors with near normal karyotypes and suggests that genomic instability may be essential in cancer development.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Chromosome Aberrations*
Diploidy
Genomic Instability*
Humans
In Situ Hybridization, Fluorescence
Karyotyping
Microsatellite Repeats
Neoplasms / genetics*

Abstract

Publication types

MeSH terms

Grants and funding